The whole genome sequencing of Clarius batrachus was carried out on Roche 454, Ion Torrent and Illumina platforms. A total of ~7.5 Gb sequencing data was generated. After performing the pre processing/ curation of data by removing adapters and trimming using various online tools, these were assembled de novo in CLC Bio Workbench V 2. The assembled contigs were then considered for in silico mining using MISA tool. Following table shows the number of microsatellites based on motif type and repeat numbers.